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KMID : 0364819760140030135
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Korean Journal of Microbiology
1976 Volume.14 No. 3 p.135 ~ p.145
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Characterizations of DNA-polymerases induced by SV40 virus infection of african green monkey kidney cells (AGMK)
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Abstract
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Confluent AGMK cells were infected by large plaque SV40 virus. Levels of DNA polymeras (α and β) were measured in the cytoplasm and the cell nucleus. The activities of DNA polymerase-α which found in both the cell nucleus and the cytoplasm were increased approximately eight folds at 48 hours after infection of SV40 virus. Only insignificant but constant amounts of DNA polymerase-β were found either in the nucleus of the SV40 infected cell or of the uninfected cell. The characteristics of the SV40 virus induced DNA polymerases were compared with that of the uninfected cellular DNA polymerase in regard of the effects of pH, salt concentration, NEM concentration and temperature on those enzyme activities. No differential effect was found between both enzymes. Endouclease activities were examined in the purified DNA polymerase-α and β. The low level of endonuclease activity which might cut SV40 DNA 1 at one site was observed in the DNA polymerase-α whereas high but nonspecific endonuclease activities were found in the DNA polymerase-β
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